It has been found that human red cell ghosts react differently in the presence of various sugars in the medium. The stability of spheric ghosts is preserved in solutions of sugars entering red cells by means of the common carrier. In media of other sugars the ghosts' shapes change to shrunken, crena
Sugar penetration into human blood platelets
โ Scribed by R. P. McDonagh Jr.; M. J. Burns; K. E. Delaimi; R. G. Faust
- Publisher
- John Wiley and Sons
- Year
- 1968
- Tongue
- English
- Weight
- 184 KB
- Volume
- 72
- Category
- Article
- ISSN
- 0021-9541
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โฆ Synopsis
The relative rates of penetration of pentoses and hexoses into human blood platelets were studied with a photoelectric densimeter. It was concluded that pentoses enter human platelets by free diffusion whereas hexoses penetrate into these cells by an altered diffusion process.
๐ SIMILAR VOLUMES
The permeability of human platelets to glycerol was determined at 37OC, 25OC, and OยฐC from the rate of change of cell volume after abrupt addition of 0.5 mol/liter glycerol in phosphate-buffered saline. lntracellular water volume was measured employing both tritiated water and a photometric method.
To measure the concentrations of cytosolic ionized calcium in platelets we used a calcium-sensitive fluorophor (Fura-2) with two different spectrofluorometers (Perkin-Elmer LS-5 and Fluorolog 222). Values obtained by these two instruments for the basal cytosolic ionized calcium concentration of rest