Sugar constituents of fucose-containing polysaccharides from various Japanese brown algae

✍ Scribed by E. Nishide; H. Anzai; N. Uchida; K. Nisizawa

• Publisher: Springer
• Year: 1990
• Tongue: English
• Weight: 267 KB
• Volume: 204-205
• Category: Article
• ISSN: 1573-5141
• DOI: 10.1007/bf00040289

✦ Synopsis

Sugar constituents of the fucose-containing polysaccharides (FCPs) from 21 species of brown algae were analyzed . FCPs were extracted with hot water (100 °C, 4 h), separated by precipitation with 20% (v : v) ethanol in the presence of 0 .05 M MgCl2 to remove contaminating soluble alginate, and purified by DEAE-Sephadex column chromatography . The samples were hydrolyzed with HCl, and neutral sugar and uronic acid were separated by anion exchange chromatography . Their amounts were determined by gas-liquid chromatography . The neutral sugars in the FCPs from Ishige okamurae, Laminaria ochotensis, Myelophycus simplex, Padina arborescens and Sargassum thunbergii all contained arabinose, fucose, galactose, glucose, mannose, rhamnose and xylose residues . The FCPs from Ishige okamurae, Padina arborescens, Sargassum hemiphyllum, S . patents and S . sagamianum contained the four uronic acids, galacturonic acid, glucuronic acid, guluronic acid and mannuronic acid .