Sugar Binding Polymers Showing High Anomeric and Epimeric Discrimination Obtained by Noncovalent Molecular Imprinting

✍ Scribed by A.G. Mayes; L.I. Andersson; K. Mosbach

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 539 KB
• Volume: 222
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1994.1521

✦ Synopsis

Noncovalent molecular imprinting of sugar compounds in ethylene glycol dimethacrylate-methacrylic acid copolymers yielded materials containing highly selective sugar binding sites. Investigation of a range of polymers demonstrated that the resulting polymer imprints have a high degree of both anomeric and epimeric selectivity favoring the original print molecule. In HPLC assays, a polymer imprinted using (p)-nitrophenyl- (\alpha)-D-galactoside could separate the (\alpha) and (\beta) anomers of the same compound with near baseline resolution. A polymer imprinted using (p)-nitrophenyl- (\beta)-D-galactoside showed similar results but with the (\beta) anomers retained longer as expected. Anomeric discrimination of closely related sugars was also possible, with the degree of separation depending on the structural resemblance to the print molecule. Similarly, a polymer imprinted with (p)-nitrophenyl- (\alpha)-L-fucoside could separate (\alpha / \beta) mixtures of p-nitrophenyl-L-fucoside with baseline resolution. Using radioligand displacement assays a polymer imprinted using octyl- (\alpha)-D-glucoside was shown to bind methyl- (\alpha)-D-glucoside with a 40 -fold higher affinity than that for the (\beta)-anomer. The epimeric selectivity was even more impressive: methyl- (\alpha)-Dmannoside and methyl- (\alpha)-D-galactoside had (130-) and 240 -fold lower affinity, respectively, than methyl- (\alpha)-Dglucoside. The results are discussed in relation to possible uses of such polymers in separation and analysis. c 1994 Academic Press. Inc.