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Sucrose-phosphate synthase activity and yield analysis of tomato plants transformed with maize sucrose-phosphate synthase

โœ Scribed by Marianne M. Laporte; Julie A. Galagan; Joseph A. Shapiro; Michael R. Boersig; Christine K. Shewmaker; Thomas D. Sharkey


Publisher
Springer-Verlag
Year
1997
Tongue
English
Weight
574 KB
Volume
203
Category
Article
ISSN
0032-0935

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Two forms of sucrose-phosphate synthase (EC 2.4.1.14) were resolved from leaves of three species, maize (Zea mays L. cv. Pioneer 3184), soybean (Glycine max (L.) Merr., cv. Ransom) and spinach (Spinacia oleracea L. cv. Resistoflay) by hydroxyapatite Ultregel chromatography, using a 75-raM (designate

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A dot-blot technique was developed using monoclonal antibodies to measure, rapidly and accurately, the amount of sucrose-phosphate synthase (SPS; EC 2.4.1.14) protein present in a crude extract from spinach (Spinacia oleracea L. cv. Dark Green Bloomsdale) leaves; this was compared with SPS activity