A dot-blot technique was developed using monoclonal antibodies to measure, rapidly and accurately, the amount of sucrose-phosphate synthase (SPS; EC 2.4.1.14) protein present in a crude extract from spinach (Spinacia oleracea L. cv. Dark Green Bloomsdale) leaves; this was compared with SPS activity
Sucrose-6-phosphate levels in spinach leaves and their effects on sucrose-phosphate synthase
β Scribed by Klaus-Peter Krause; Mark Stitt
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 517 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0031-9422
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Two forms of sucrose-phosphate synthase (EC 2.4.1.14) were resolved from leaves of three species, maize (Zea mays L. cv. Pioneer 3184), soybean (Glycine max (L.) Merr., cv. Ransom) and spinach (Spinacia oleracea L. cv. Resistoflay) by hydroxyapatite Ultregel chromatography, using a 75-raM (designate