Successful expression in pollen of various plant species ofin vitrosynthesized mRNA introduced by particle bombardment
✍ Scribed by Toshinori Tanaka; Masahiro Nishihara; Motoaki Seki; Atsushi Sakamoto; Kunisuke Tanaka; Kohei Irifune; Hiromichi Morikawa
- Publisher
- Springer
- Year
- 1995
- Tongue
- English
- Weight
- 404 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0167-4412
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✦ Synopsis
Gold particles coated with fl-glucuronidase (GUS) mRNA with a 5' cap structure that had been synthesized in vitro were introduced, by use of a pneumatic particle gun, into pollen grains of lily (Lilium longiflorum), freesia (Freesia refracta) and tulip (Tulipa gesneriana). A fluorometric assay for the GUS activity indicated that in vitro synthesized GUS mRNA introduced into these pollen cells by particle bombardment was successfully expressed. GUS activity in extracts of the bombarded lily pollen became detectable ftuorometrically within 30 rain after bombardment, peaked at 6 h, then gradually decreased. This activity changed as a function of the developmental stage of the pollen cell of lily.