Subtractive hybridization, a technique for extraction of DNA sequences distinguishing two closely related genomes: critical analysis

Application of a subtraction hybridizati

Application of a subtraction hybridization technique involving photoactivatable biotin and organic extraction to solution hybridization analysis of genomic DNA

✍ Frederic G. Barr; Beverly S. Emanuel 📂 Article 📅 1990 🏛 Elsevier Science 🌐 English ⚖ 708 KB

We have adapted a subtraction hybridization technique involving photoactivatable biotin, streptavidin binding, and organic extraction for solution hybridization analysis of mammalian genomic DNA. By combining maximal hybridization conditions of high salt, dextran sulfate, and formamide with successi