𝔖 Bobbio Scriptorium
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Substrates for the assay of α-l-iduronidase

✍ Scribed by Jerry N. Thompson


Book ID
115822347
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
893 KB
Volume
89
Category
Article
ISSN
0009-8981

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A method for α-L-iduronidase assay
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Properties of α-l-iduronidase in culture
✍ S. Fujibayashi; R. Minami; Y. Ishikawa; K. Wagatsuma; T. Nakao; S. Tsugawa 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 324 KB

On DEAE cellulose column chromatography, alpha-L-iduronidase in cultured skin fibroblasts was resolved into two distinct components, forms A and B. They had similar Km values for 4-methylumbelliferyl-alpha-L-iduronide, but differed in pH optima and thermal stability. Form B was more heat-stable than