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Substrate specificity of the CYC2 enzyme from Kitasatospora griseola: production of sclarene, biformene, and novel bicyclic diterpenes by the enzymatic reactions of labdane- and halimane-type diterpene diphosphates

✍ Scribed by Chiaki Nakano; Tsutomu Hoshino; Tsutomu Sato; Tomonobu Toyomasu; Tohru Dairi; Takeshi Sassa


Book ID
104097114
Publisher
Elsevier Science
Year
2010
Tongue
French
Weight
242 KB
Volume
51
Category
Article
ISSN
0040-4039

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✦ Synopsis


The enzymatic reaction of copalyl diphosphate (CDP), ent-CDP, syn-CDP, and tuberculosinyl diphosphate with the CYC2 enzyme from Kitasatospora griseola afforded sclarene, biformene, and novel diterpenes having a buta-1,3-diene moiety in the side chain. The substrate specificity of the CYC2 is discussed.