Substrate specificities and inhibition of intra cellular alkaline phosphatases associated with cytoplasmic granules sedimented by differential centrifugation
✍ Scribed by Kallman, Frances Green
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1951
- Tongue
- English
- Weight
- 933 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0095-9898
No coin nor oath required. For personal study only.
✦ Synopsis
Intracellular particles may be divided in general into two groups on the basis of sedimentation differences; the large or microscopic granules, usually considered to include the mitochondria, and the submicroscopic granules including so-called "microsomes. " Many investigators have been concerned with determining the properties of such sedimentable fractions but difficulty arises in comparing results since various tissues, various suspending media and various centrifugal forccs have becn employed. The microscopic granule fraction appears to be the most important site of localization of oxidizing enzymes such as succinic dehydrogenase and cytochrome oxidase as demonstrated in the work of Hogeboom, Claude, and Hotchkiss ( '46), Schneider ( '46), and Hogeboom, Schneider, and Pallade ( '48). Several enzymes have been demonstrated to be associated with the submicroscop;c granule fraction such as adenosine triphosphatase in chick embryos (Stein-