Substrate screening of protein kinases: Detection methods and combinatorial peptide libraries
β Scribed by Mira Kim; Dong-Sik Shin; Jaehi Kim; Yoon-Sik Lee
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2010
- Tongue
- English
- Weight
- 904 KB
- Volume
- 94
- Category
- Article
- ISSN
- 0006-3525
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Affinity chromatography using immobilized Sprotein was used for the screening of affinity peptide ligands from two soluble peptide libraries. Peptide library I consisted of octamers with glycine (G) at both termini of each peptide, i.e. GXXXXXXG. The six center positions were constructed using rando
## Abstract For Abstract see ChemInform Abstract in Full Text.
## Capillary electroseparations of enkephalin-related peptides and protein kinase A peptide substrates The separations of enkephalin-related peptides and protein kinase A peptide substrates, with the common structural feature -Arg-Arg-X-Ser-Val-, were studied in micellar electrokinetic chromatogra