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Subfractionation of the dansylated derivatives of glucosyl galactosyl hydroxylysine by liquid chromatography and its application to a specific α-1,2-glucosidase assay

✍ Scribed by Hitoo Iwase; Ikuko Ishii; Yukinobu Kato; Hideaki Hamazaki; Kyoko Hotta; Akira Umezawa; Tamotsu Kanzaki


Book ID
103803450
Publisher
Elsevier Science
Year
1987
Weight
517 KB
Volume
416
Category
Article
ISSN
0378-4347

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A sensitive and selective determination of a-keto acids was established by the use of a gas chromatograph equipped with an electron capture detector. a-Keto acids (pyruvic, oxaloacetic, a-ketobutyric, and a-ketoglutaric acids) were reacted with pentafluorophenylhydrazine, and the derivatives were ex