Subcutaneous implantation of human post-transplant lymphoproliferative disease lesions in SCID mice
✍ Scribed by Parmjeet S. Randhawa; Beatta Pietrzak; Michael A. Nalesnik; Anthony Jake Demetris; Joseph Locker
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 67 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0278-0232
No coin nor oath required. For personal study only.
✦ Synopsis
The tumorigenic potential of six post-transplant B-cell lymphoproliferative disease (PTLD) lesions was evaluated in SCID mice. Three animals developed local subcutaneous B cell tumours, two of which contained Epstein-Barr virus (EBV) DNA. Two animals developed CD3 positive thymic neoplasms, and one mouse developed an uncharacterized spontaneously regressing subcutaneous tumour. Immunoglobulin gene rearrangements studies with a JH probe, and EBV clonality studies with a Bam NJ fused terminal probe, showed only one mouse tumour to be genealogically related to the corresponding clinical lesion. It is concluded that lymphoid clones which constitute human PTLD are not autonomous, but sustained by host-derived growth stimuli distinct from those operating in the SCID mouse mileu. 1997 John Wiley & Sons, Ltd.