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Subculturing monolayer cell cultures without the use of centrifugation

โœ Scribed by E. P. Ugryumov


Publisher
Springer US
Year
1965
Tongue
English
Weight
142 KB
Volume
59
Category
Article
ISSN
0007-4888

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High density culture of hybridoma cells
โœ Michiyuki Tokashiki; Takami Arai; Kimihiko Hamamoto; Kenji Ishimaru ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Springer ๐ŸŒ English โš– 368 KB

The influence of centrifugal force on the growth of cells was examined by exposing the cells of the mouse-human hybridoma X87 line to centrifugal force (100-500 G) for ten minutes twice a day and comparing the static culture with that of unexposed cells. In this experiment, both cell proliferation a