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Subcellular Quantification of Doxorubicin and Its Metabolite in Cultured Human Leukemia Cells Using Liquid Chromatography-Tandem Mass Spectrometry

✍ Scribed by Xu, Jinhui; Liu, Yuan; Yu, Ying; Ni, Qingjiang; Chen, Yun


Book ID
127254031
Publisher
Taylor and Francis Group
Year
2012
Tongue
English
Weight
361 KB
Volume
45
Category
Article
ISSN
0003-2719

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A validated method is described for the simultaneous analysis of PGE 2 , 11-, 12-, and 5-HETEs from cultured cells using HPLC negative electrospray ionization tandem mass spectrometry (LC/MS/MS). This method permits quantification of selected individual arachidonic acid metabolites from cell extract