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Subcellular localization of phospholipids and enzymes involved in PAF-acether metabolism

✍ Scribed by Michel Record; Gérard Ribbes; François Tercé; Hugues Chap

Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
449 KB
Volume
40
Category
Article
ISSN
0730-2312

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✦ Synopsis

The biosynthesis of platelet-activating factor (PAF-acether or 1 -0-alkyl-2-acetylsn-glycerc-3-phosphoholine) through the remodeling pathway was investigated at the subcellular level in two different cell lines. In human neutrophils, plasma membrane was isolated not only from granules, but also from internal membranes related to endoplasmic reticulum. Interestingly, the latter exhibited enhanced acetyltransferase upon neutrophil stimulation with ionophore A similar study was undertaken on the tumor strain Krebs-I1 cells. The enzyme acetyltransferase was found to be located only on an endoplasmic reticulum subfraction, whereas most alkylacyl-GPC, the source of PAF-precursor alkyl-lyso-GPC, was located in the plasma membrane inner leaflet. The topographical separation of enzyme and precursor emphasizes the central role of the intracellular phospholipase A, in providing lyso-PAF to the acetyltransferase to form PAF-acether.

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