Subcellular Fractionation: A Practical Approach (Practical Approach Series)

✍ Scribed by John Graham, David Rickwood

• Year: 1997
• Tongue: English
• Leaves: 360
• Edition: 1
• Category: Library

✦ Synopsis

Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers all the necessary aspects, from the homogenization of cells via a variety of separation techniques, to the isolation and characterization of peroxisomes and other organelles. Basic separation techniques needed in all cell biology laboratories are given and presented in the context of a number of related topics such as the labelling of ligands, the kinetics of their internalization and analysis of data, and the electron microscopy of organelles. Subcellular Fractionation contains expert advice and detailed methodology, and will be invaluable to researchers in this important area.

✦ Table of Contents

Cover......Page 1
Subcellular Fractionation: A Practical Approach......Page 6
Preface......Page 8
Contents......Page 10
Contributors......Page 18
Abbreviations......Page 20
1. Homogenization of tissues and cells......Page 22
2. Isolation of subcellular fractions......Page 52
3. Isolation and characterization of nuclei and nuclear subfractions......Page 92
4. Subcellular fractionation of mitochondria......Page 128
5. Isolation and characterization of peroxisomes......Page 164
6. Lysosomes and endocytosis......Page 190
7. The membranes of the secretory and exocytic pathways......Page 226
8. Isolation and purification of functionally intact mitochondria and chloroplasts from plant cells......Page 264
9. Ribosomes and polysomes......Page 292
10. Electron microscopy of organelles......Page 324
A1. List of suppliers......Page 350
Index......Page 354