Subcellular distribution of yeast invertase isoenzymes
β Scribed by J. Meyer; Ph. Matile
- Publisher
- Springer
- Year
- 1975
- Tongue
- English
- Weight
- 720 KB
- Volume
- 103
- Category
- Article
- ISSN
- 0302-8933
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β¦ Synopsis
Homogenates from yeast cells contain 1 ~ or less vacuoles whereas the small isoenzyme is largely present in of sedimentable invertase activity. Sedimentability is the soluble cell fraction. These findings indicate that equally low in homogenates from cells repressed or de-vesicles are not involved in the secretion of invertase. A repressed with regard to invertase secretion. Intracellularly, soluble mode of invertase secretion is discussed. the mannanprotein form of invertase is largely localized in
π SIMILAR VOLUMES
A method for the rapid isolation of yeast enolases, yielding three distinct isoenzymes, has been devised. In the first step anionic proteins were precipitated with polyethyleneimine, whereas hydrophobic enolase isoenzymes remained in the supernatant. Secondly, the supernatant was 45% saturated with