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Subcellular distribution of ubiquitin–protein conjugates in the hippocampus following transient ischemia

✍ Scribed by Dr. T. Hayashi; K. Takada; M. Matsuda

Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 426 KB
Volume: 31
Category: Article
ISSN: 0360-4012
DOI: 10.1002/jnr.490310321

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✦ Synopsis

Ubiquitin-protein conjugates in the hippocampus were analyzed by immunoblotting with a monoclonal anti-ubiquitin antibody. In the CA1 region, Triton X-100 insoluble ubiquitin-protein conjugates increased after 24 hr following 20 min of ischemia. When the total hippocampi were fractionated subcellularly , ubiquitin-protein conjugates increased in the particulate, especially in the mitochondria1 fraction. The ubiquitin-protein conjugates were solubilized by SDS, or were partially solubilized by urea. The results indicate that insoluble ubiquitin-protein conjugates increase after ischemia.

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