Sub-Parts-per-Million Spectrophotometric Determination of Phenol and Related Pesticides Using Diazotizedp-Aminoacetophenone
✍ Scribed by Omi Agrawal; V.K. Gupta
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 35 KB
- Volume
- 62
- Category
- Article
- ISSN
- 0026-265X
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✦ Synopsis
A spectrophotometric determination of phenol based on the coupling with diazotized p-aminoacetophenone in alkaline medium is described. The dye formed is measured at 475 nm against the reagent blank. Beer's law is obeyed over the concentration range 0.6 -6.0 g per 25 ml of final solution (0.024 -0.24 ppm). Molar absorptivity and Sandell's sensitivity were found to be 3.13 ϫ 10 5 liters mol Ϫ1 cm Ϫ1 and 0.0003 g cm Ϫ2 , respectively. The method has been applied to the determination of phenol and phenol-liberating pesticides, i.e., carbaryl, propoxur, carbofuran, and fenthion, after their thin-layer chromatographic separation in environmental and biological samples. The method is free from of interference from various organic compounds and inorganic ions.