Sub-2 μm HPLC coupled with sub-ppm mass accuracy for analysis of pharmaceutical compound libraries

✍ Scribed by Justin G. Stroh; Christopher J. Petucci; Scott J. Brecker; Lisa M. Nogle

• Book ID: 102441726
• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 487 KB
• Volume: 31
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200800399

✦ Synopsis

Abstract

Recent advances in accurate mass analysis are poised to allow the high‐throughput production of accurate mass data on many more compounds than was previously available. It is shown that sub‐ppm mass accuracy (producing elemental compositions) can be obtained on a simple TOF mass spectrometer operating in the manufacturer's standard mode. Concomitantly, there have been important technological advances in LC with respect to speed of analysis using sub‐2 μm particle columns. Much of the sub‐2 μm work in the literature has been under the label ultra performance LC (UPLC), however, we show that very high‐speed results can be obtained using other manufacturer's pumps by using elevated column temperatures. Using elevated temperatures, HPLC peak widths on the order of 1 s can be obtained. We report the coupling of these two technologies (sub‐ppm mass accuracy MS with high‐speed HPLC) for the rapid analysis of compounds entering pharmaceutical libraries.