Styryl-Based Wavelength-Ratiometric Probes: A New Class of Fluorescent Calcium Probes with Long Wavelength Emission and a Large Stokes′ Shift
✍ Scribed by E.U. Akkaya; J.R. Lakowicz
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 403 KB
- Volume
- 213
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
We describe the chemical synthesis and fluorescence spectral characterization of a styryl-benzothiazole probe which contains the (\mathrm{Ca}^{2+}) chelating group BAPTA as an integral part of the chromophoric system. The visible absorption spectrum of this probe displays a dramatic shift in the long wavelength maxima from 508 to (407 \mathrm{~nm}) upon complexation with (\mathrm{Ca}^{2+}), with a (\mathrm{Ca}^{2+}) dissociation constant of (1.5 \mu \mathrm{M}). The emission maximum centered at (615 \mathrm{~nm}) is well shifted from the absorption. The emission spectrum displays a small blue shift upon binding (\mathrm{Ca}^{2+}), allowing this probe to possibly be used as an emission wavelength-ratiometric probe using a singleexcitation wavelength. This probe is likely to be the first of a series of long-wavelength ratiometric (\mathrm{Ca}^{2+}) probes whose structure can be modified for improved quantum yield or altered (\mathrm{Ca}^{2+}) affinity. 1993 Academic Press, Inc.