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Studying DNA mutations in human cells with the use of an integrated HSV thymidine kinase target gene

✍ Scribed by Micheline Giphart-Gassler; Andrea Groenewegen; Hans den Dulk; Pieter van de Putte; Judith G. Tasseron-de Jong


Book ID
113193680
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
812 KB
Volume
214
Category
Article
ISSN
0027-5107

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## Abstract To analyze the site of integration of the herpes simplex virus type 1 (HSV‐1) thymidine kinase (TK) gene in biochemically transformed human cells, TK‐HeLa‐(BU25) cells were transformed to the TK^+^ phenotype by a cloned, 2 kbp Pvull fragment of HSV‐1 DNA. The transformed cells [HeLa(BU2