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πŸ“

Studying Cell Metabolism and Cell Interactions Using Microfluidic Devices Coupled with Mass Spectrometry

✍ Scribed by Huibin Wei (auth.)


Publisher
Springer-Verlag Berlin Heidelberg
Year
2013
Tongue
English
Leaves
116
Series
Springer Theses
Edition
1
Category
Library

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✦ Synopsis


This thesis describes a new approach for cell analysis by the rapid developing microfluidic technology. The nominee has made great contributions to develop a new analysis platform which combined microfluidic devices with mass spectrometry to determine the trace compounds secreted by cells. Based on this analysis platform, she studied the specific cell secreting behaviors under controlled microenvironment, of which the secretion compounds were qualified and semi-quantified by mass spectrometry. A novel cell sorting device integrated homogenous porous PDMS membrane was invented to classify cells from real samples based on the size difference. The nominee further studied the signal transmission between different cells, and the signal chemicals were qualitative and quantitative monitored by the analysis platform. This indicates the potential significant application of the new cell analysis platform in medicine screening and early diagnosis.

✦ Table of Contents


Front Matter....Pages i-xvi
Introduction....Pages 1-25
Analysis of Herbicides on a Single C30 Bead via the Platform Combined Microfluidic Device with ESI-Q-TOF-MS....Pages 27-41
Monitoring of Glutamate Release from Neuronal Cell Based on the Analysis Platform Combining the Microfluidic Devices with ESI-Q-TOF MS....Pages 43-60
Microfluidic Device with Integrated Porous Membrane for Cell Sorting and Separation....Pages 61-82
Cell Co-culture and Signaling Analysis Based on Microfluidic Devices Coupling with ESI-Q-TOF MS....Pages 83-102

✦ Subjects


Mass Spectrometry; Nanotechnology and Microengineering; Cell Biology; Analytical Chemistry


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