Study on the synthesis of sucrose-6-acetate catalyzed by fructosyltransferase from Aspergillus oryzae

✍ Scribed by Yawei Han; Guoming Liu; Dongye Huang; Baojian Qiao; Liping Chen; Lihong Guan; Duobin Mao

• Publisher: Elsevier
• Year: 2011
• Tongue: English
• Weight: 320 KB
• Volume: 28
• Category: Article
• ISSN: 1871-6784
• DOI: 10.1016/j.nbt.2010.07.007

✦ Synopsis

The study had mainly investigated the synthesis of sucrose-6-acetate (s-6-a) in fructosyltransferase action. The synthesis reaction of s-6-a was performed between sucrose and glucose-6-acetate (g-6-a), and identified by high performance liquid chromatography (HPLC). According to the reaction of s-6-a catalyzed by fructosyltransferase from Aspergillus oryzae, the effect factors of reaction, such as the ratio of g-6-a to sucrose, temperature, time, pH, substrate and enzyme concentration in the reaction, were investigated. All results indicated that the fructosyltransferase could catalyze the s-6-a synthesis, and the optimal conditions of fructosyltransferase in reaction were 50°C, pH 6.2, 48h reaction time, 60% sucrose, 1:3 ratio of g-6-a to sucrose and 4.0mg/L concentration of enzyme. This study plays the important role in sucralose synthesis, because it is very cumbersome in the reported methods.