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Study on the scale-up of human IgG3 purification using protein A affinity chromatography

✍ Scribed by J. Amaral; M. Inganäs; J. Cabral; D. Prazeres

Book ID
110347641
Publisher
Springer
Year
2001
Tongue
English
Weight
322 KB
Volume
10
Category
Article
ISSN
1573-8272

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✍ Leda R Castilho; Wolf-Dieter Deckwer; F.Birger Anspach 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 121 KB

Nylon microfiltration membranes were coated with dextrans (M r ≈ 6000 and 40,000) and poly(vinylalcohol) (PVA) (M r ≈72,000) to produce affinity matrices with adequate chemical functionality and low non-specific protein binding characteristics. In all cases, a considerable reduction of non-specific