Study on the decomposition of the amadori compound N-(1-deoxy-D-fructos-1-y1)-glycine in model systems: Quantification by fast atom bombardment tandem mass spectrometry
✍ Scribed by Andreas A. Staempfli; Imre Blank; René Fumeaux; Laurent B. Fay
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 408 KB
- Volume
- 23
- Category
- Article
- ISSN
- 1076-5174
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✦ Synopsis
A new method for the quantification of N-(l-deoxy-D-fructos-l-yl)-glycine (DFG) was developed based on isotope dilution fast atom bombardment tandem mass spectrometry using 13C-labelled DFG as an internal standard. This method, which requires neither derivatization nor clean-up of the samples, was used to study the degradation of DFG under different conditions (time and pH). It was found that the decomposition of DFG at 90°C was favoured at pH 7 compared to pH 6. The higher stability of DFG at pH 6 was due to 1,2enoIization leading to the relatively stable 3-deoxglucosone.