Study on PEG-(NH4)2SO4 aqueous two-phase system and distribution behavior of drugs

✍ Scribed by Lei Li; Chi-Yang He; She-Hong Li; Feng Liu; Shun Su; Xiang-Xu Kong; Na Li; Ke-An Li

• Book ID: 102804092
• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 509 KB
• Volume: 22
• Category: Article
• ISSN: 0256-7660
• DOI: 10.1002/cjoc.20040221120

✦ Synopsis

Abstract

The distribution behavior of chlorpromazine hydrochloride (CPZ), procaine hydrochloride (PCN) and procaine amide hydrochloride (PCNA) in polyethylene glycol (PEG800 or PEG1500)‐(NH~4~SO~4~ aqueous two‐phase systems has been investigated. The result shows that the PEG‐(NH~4~SO~4~ aqueous two‐phase system has potential extraction capability in small molecular drug separation. In PEG800–(NH~4~)~2~SO~4~ system, the extraction efficiencies (E) of CPZ, PCN and PCNA amount to 92.8%, 74.5% and 74.4%, respectively, with the distribution coefficients (K~D~) being 25.7, 5.9 and 5.8, correspondingly. In PEG1500–(NH~4~)~2~SO~4~ system, the extraction efficiencies (E) of CPZ, PCN and PCNA are 93.7%, 71.3% and 63.24, respectively, with distribution coefficients (K~D~) of 39.6, 6.6 and 5.0, correspondingly. Based on the study on ultraviolet and fluorescence spectra and also distribution behavior of the drugs in PEG‐(NH~4~)SO~4~ aqueous two‐phase system, extraction mechanism was further proposed that both hydrogen bond and hydrophobic interaction are involved in extraction.