Study of the thymocyte cell cycle by bivariate analysis of incorporated bromodeoxyuridine and DNA content
✍ Scribed by Christophe Baron; Claude Pénit
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 576 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0014-2980
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Ribonucleotide reductase (RR) is a cytoplasmatic enzyme catalyzing the reduction of all four ribonucleotides to their corresponding deoxyribonucleotides. Its activity strongly correlates to the rate of DNA synthesis. By using a specific monoclonal antibody against the large M1 subunit of RR, we asse
This study compares the cell cycle distribution in rat thymocytes obtained by means of bromodeoxyuridine (BrdUrd) labeling of S-phase cells and the analysis of the S-phase fraction obtained according t o the technique ofvindelov et al. (Cytometry 3332-338,1983). The proportion of BrdUrd-labeled cell