𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Study of the circular dichroism of bacteriophage ϕ6 and ϕ6 nucleocapsid

✍ Scribed by H. Thomas Steely Jr.; Donald M. Gray; Dimitrij Lang

Book ID
102765945
Publisher
Wiley (John Wiley & Sons)
Year
1986
Tongue
English
Weight
985 KB
Volume
25
Category
Article
ISSN
0006-3525

No coin nor oath required. For personal study only.

✦ Synopsis

We have studied the CD of both bacteriophage 4 6 and 4 6 nucleocapsid in order to assess the in situ state of the viral, double-stranded RNA (dsRNA). The results showed that packaged (p6 RNA is slightly hyperdichroic ( 4 4 % ) at 264 nm relative to dsRNA in solution. Also, the CD of dsRNA within both the virus and the nucleocapsid was unlike CD spectra of any of the three types of dsRNA condensed in uitro, as described in the accompanying paper. Using a curve-fitting program described elsewhere (Edmondson, S.P. & Gray, D.M. ( 1983) Nucleic Acids Res. 11,[175][176][177][178][179][180][181][182][183][184][185][186][187][188][189][190][191][192], we have fit reference CD spectra of (p6 RNA and protein to measured CD spectra of $6 intact phage and nucleocapsid. Results of the computer analysis indicated that 46 RNA in situ is spectrally similar to dsRNA that is slightly dehydrated.

📜 SIMILAR VOLUMES

Assignment of viral proteins to the thre
Assignment of viral proteins to the three double-stranded RNA segments of bacteriophage ϕ6 genome: Translation of ϕ6 messenger RNAs transcribed in vitro
✍ Emori, Yasufumi ;Iba, Hideo ;Okada, Yoshimi 📂 Article 📅 1980 🏛 Springer 🌐 English ⚖ 471 KB

Pseudomonas phaseolicoIa bacteriophage qb6 has a double-stranded (ds) RNA genome in three segments (L, M, S) which can serve as templates for in vitro transcription by 46 nucleocapsid. Singlestranded (ss) RNA (l, m, s) synthesized in vitro functioned as messenger RNA of viral proteins in an Escheric