Study of the Adsorption of Some Amino Acids by Silica Chemically Modified with Aminobenzenesulfonic and Phosphate Groups

✍ Scribed by Lauro T. Kubota; Alessandra Gambero; Antonio Santana Santos; José M. Granjeiro

• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 122 KB
• Volume: 183
• Category: Article
• ISSN: 0021-9797
• DOI: 10.1006/jcis.1996.0568

✦ Synopsis

and elution processes because they affect the net charge and Two silica gels, one modified with aminobenzenesulfonic complementary interaction between mobile and stationary (SABS) groups and the other with phosphate (SZP) groups, were phases (17, 18). Amino acids may be very useful in investiprepared to adsorb some amino acids. Chemical analysis of the gating these properties because of their ionic equilibria.

modified silica gave 0.65 mmol g 01 aminobenzenesulfonic groups Some proteins have affinity with phosphate and sulfonic and 0.56 mmol g 01 phosphate groups. The maximum adsorption groups (19); thus, studies of amino acid interaction with capacities for amino acids determined by batch experiments for these groups are very important to aid the understanding of SABS were 1.37, 0.67, 0.76, and 0.59 mmol g 01 for glycine, lysine, this characteristic process and the use of this material as a histidine, and leucine, respectively, and those for SZP were 0.75, stationary phase for amino acid and protein separation (20). 0.58, 0.44, and 0.75 mmol g 01 for glycine, lysine, histidine, and leucine, respectively. The adsorption capacity of SABS was sig-

The preparation of a silica gel modified with sulfonic nificantly affected by the solution pH, showing a higher selectivity groups and another with phosphate groups is described. than SZP. The materials were very stable, allowing their use sev-Amino acid adsorption by this material was studied. A comeral times without changes in adsorption capacity. ᭧ 1996 Academic parison between them is also reported in this work.