Study of the adsorption of albumin on a platinum rotating disk electrode using impedance measurements

✍ Scribed by P. Bernabeu; L. Tamisier; A. De Cesare; A. Caprani

• Publisher: Elsevier Science
• Year: 1988
• Tongue: English
• Weight: 746 KB
• Volume: 33
• Category: Article
• ISSN: 0013-4686
• DOI: 10.1016/0013-4686(88)80204-4

✦ Synopsis

The study of the adsorption of albumin on a platinum rotating disk electrode was performed using impedance measurements. The kinetics of the adsorption was investigated by recording, during 15 min after protein addition, the variation with time of the electrochemical double layer capacitance, Cd. The resulting curve was computed and a model of two consecutive reactions occurring at the interface was proposed to describe the adsorption reaction. The corresponding theoretical equation used to fit the data showed five parameters: three capacitances (of the bare surface, of the surface covered with the reversibly adsorbed proteins,andofthesurfacecovered with theirreversiblyadsorbedproteins)and two timeconstants. The influence of the shear rate on these parameters was studied within the range O-1728 s-' and the two time constants, corrected for the mass transfer contribution, showed a shear dependence. Different nossibilities were considered for the decrease in canacitance that goes with the adsorbed protein denaturation reaction.