## Methods An HPLC procedure has been developed that can be RNA phosphoramidites and supports, 6-FAM phosused to monitor the rate of phosphodiester cleavage of phoramidite, and ancillary reagents for automated synan oligoribonucleotide substrate by an RNA ribozyme theses were obtained from Applied
Study of protein reactivity with thiol reagents by anion-exchange high-performance liquid chromatography
β Scribed by J.P. Mahieu; B. Sebille; M. Gosselet; M.C. Garel; Y. Beuzard
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 813 KB
- Volume
- 359
- Category
- Article
- ISSN
- 1873-3778
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That the macroporous anion-exchange resin AG MP-1 can be used with HPLC equipment and common aqueous buffers for the chromatography of proteins is shown. The utility of this system is illustrated by the partial purification and complete resolution of the three protein synthesis elongation factors fr
Phosphatidylinositol 4,5-biphosphate (PIP 2 ) modulates the function of numerous ion transporters and channels, as well as cell signaling and cytoskeletal proteins. To study PIP 2 levels of cells without radiolabeling, we have developed a new method to quantify anionic phospholipid species. Phosphol
## Asymmetrical hybrid hemoglobins formed from mixtures of oxyhemoglobins S and F and A and F were separated by high-performance liquid chromatography on a 4.6 X 250 mm wide-pore polyethyleneimine-silica gel column under anaerobic conditions. The resulting HPLC chromatogram showed three peaks, wit