Study of different coupling agents in the conjugation of a V3-based synthetic MAP to carrier proteins
✍ Scribed by Luis J. Cruz; Enrique Iglesias; Julio C. Aguilar; Diogenes Quintana; Hilda E. Garay; Carlos Duarte; Osvaldo Reyes
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 588 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1075-2617
- DOI
- 10.1002/psc.336
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✦ Synopsis
Abstract
The conjugation of synthetic peptides to carrier proteins is a widely used method for immunological studies. Different coupling agents have been described to form the conjugate with carrier proteins. In this paper, we demonstrate that the antibody response toward V3‐based synthetic MAPs derived from HIV‐1, JY1 isolate, conjugated to two different carrier proteins using either m‐maleimidobenzoyl‐N‐hydroxysuccinimide ester (MBS) or β‐maleimidopropionic acid N‐hydroxysuccinimide ester (MPS), or succinic anhydride (SA) show different behaviors. An excellent anti‐JY1 response without a strong response to the coupling agent is observed in the case of succinic anhydride spacer. In contrast, MBS produces total abrogation of the antibody response with a high response toward the coupling agent. Copyright © 2001 European Peptide Society and John Wiley & Sons, Ltd.