## Abstract Human parvovirus B19 (B19) encodes a number of nonstructural proteins, including the major protein, NS1, and two structural proteins, VP1 and VP2. The use of denatured NS1 in enzyme‐linked immunosorbent assay (ELISA) and Western blot (WB) assay has provided an opportunity to study some
Study of anti-dengue NS1 antibody by Western blot
✍ Scribed by G. Kuno; A. V. Vorndam; D. J. Gubler; I. Gómez
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 653 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0146-6615
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
The presence of anti‐nonstructural protein (NS1) antibody in natural dengue infections has been suspected to be associated with development of dengue haemorrhagic fever (DHF). The Western blot technique was used to study the dynamics of the immune response to NS1 and to determine the frequency of anti‐NS1 antibody among confirmed dengue patients in Indonesia, where DHF is common, and in Puerto Rico, where DHF is less frequently observed. Anti‐NS1 antibody was rarely found in those with primary infections in either group. The antibody occurred at a significantly higher frequency in acute‐phase serum samples from secondary infections in Indonesia than in those from Puerto Rico. No difference was observed, however, in Indonesian patients with secondary infection who had dengue fever or DHF.
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