Study of a plane-free jet exhausting from a channel by vortex-in-cell method

A rapid method to study heterogeneous ge

A rapid method to study heterogeneous gene expression in liver by direct assay of messenger RNA from periportal and perivenous cell lysates

✍ Juhani Saarinen; Ritva Saarelainen; Kai O. Lindros 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 435 KB

To study zonation of liver gene expression, we obtained periportal or perivenous rat liver cell lysates virtually devoid of nuclear material by site-directed digitonin infusion in situ. Total RNA was isolated, messenger RNAs were reverse transcribed and complementary DNAs were assayed after polymera