Study by the isotopic antiglobulin technique of a cross-reacting murine antibody produced by immunization with a syngeneic SV40 tumor
✍ Scribed by James F. Burdick; Dale Stemple; Samuel A. Wells Jr.
- Publisher
- John Wiley and Sons
- Year
- 1973
- Tongue
- French
- Weight
- 727 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Tumor line SV‐AL/N, derived from SV40 transformation of an embryonic cell line, was employed to immunize syngeneic mice, and the resultant antisera were investigated with the modified isotopic antiglobulin assay. A single specificity was detected in the antiserum, which reacted strongly with an antigen present on SV40‐transformed lines. However, the antigen was also present on cells derived from the same embryonic line as SV‐AL/N but transformed instead either spontaneously or by polyoma virus. The antigen was present on one non‐transformed syngeneic embryonic line and may have been present in low concentration in a non‐transformed syngeneic fibroblast line; it was not detected in several methylcholanthrene‐induced lines. Antiserum derived from multiple immunizations with irradiated syngeneic fetuses reacted weakly in a similar pattern. The results suggest that the antigen detected is present in the host genome in occult form, and is derepressed by several processes, perhaps including embryogenesis.