Studies with radiophosphorus in Drosophila. III. The lethal effect of P32-treatment upon developing flies
✍ Scribed by King, Robert C.
- Publisher
- John Wiley and Sons
- Year
- 1954
- Tongue
- English
- Weight
- 720 KB
- Volume
- 126
- Category
- Article
- ISSN
- 0022-104X
No coin nor oath required. For personal study only.
✦ Synopsis
It has been demonstrated that the phosphorns content of insects increases during development (Teissier, '31 : Akao, ' 3 2 ) and tliat a certain phosphorus level must be attained before papation can occur (Selson and Palmer, '3<3). Radioactive phosphorus provides a useful tool in studies of phosphorus nptake during insect development. This study deals with the effect of various concentrations of radiopliosi~liorns in the ineclinm upon development of ~l -o s o p h i ~n .
XETHODS
BIeclinm labeled with P32 is made according to the formula slio-rvn in table 1. Some of the properties of the medium as calculated from data available from analyses of the ingredients supldiecl by the inanufactuuers are shown in this table. The sum of the phosphorus contributions of the ingrcdicnts agrees with our analysis of the total phosphorus of the mel A part of tlliq stiidv was completed a t Tale T'niTersitr, rlnrixig $ 1 ~ tsiti.;i-,,T it Pubiie i h ltlr Srrvice-Predoctorate-Rachelielor Research Fellowship from the Nation:il Canter Jnstitute. Some of t h i s mnterinl was presmted in p i tial fulfilli t i n i t of the recluirenients for the degree of P1i.D. at Yale T'nirersitr. Additioiial cl:rta has been collected during the author's stay a t the Biology I)epartment o f the Broohliawii It-ational Lnborntory Liiidcr the :ruspic.r.; of the Ti. S. Atotiiic Eiiergy Co~~iniission.