Studies using a recombinant vaccinia virus expressing the circumsporozoite protein of Plasmodium berghei
β Scribed by Vijaya Satchidanandam; Fidel Zavala; Bernard Moss
- Book ID
- 113260899
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 890 KB
- Volume
- 48
- Category
- Article
- ISSN
- 0166-6851
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The DNA coding for the circumsporozoite protein (CPS) of Plasmodium falciparum has been cloned into the baculovirus expression vector pAcYM1 and expressed in Spodoptera frugiperda (Sf9) insect cells. Three DNA constructs have been made: the first one directs the synthesis of the complete CSP (aa 1-4
A polypeptide of 69 amino acids (PbCS 242-310) encompassing the C-terminal region of the circumsporozoite protein of Plasmodium berghei (PbCS) was generated using solid-phase peptide synthesis. The immunological and protective properties of peptide PbCS 242-310 were studied in BALB/c mice (H-2d). Tw