Studies on yeast host strains for heterologous P450 expression

✍ Scribed by Gang Fang; K. Venkateswarlu; Diane E. Kelly; Steven L. Kelly

• Publisher: Springer Netherlands
• Year: 1994
• Tongue: English
• Weight: 315 KB
• Volume: 16
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00149627

✦ Synopsis

The Saccharomyces cerevisiae strain AH22 was capable of human P4501Al expression without detectable background of yeast P450, unlike ATCC44773. Repeated backcrossing to AH22 produced a strain allowing transformation by vectors carrying various common selectable markers. Background yeast xenobiotic metabolism was observed only with growth on complex medium.