Studies on thePseudomonas aeruginosaPAO1bacteriophage receptors

✍ Scribed by I. R. Patel; K. K. Rao

• Book ID: 104761363
• Publisher: Springer
• Year: 1983
• Tongue: English
• Weight: 276 KB
• Volume: 135
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00408026

✦ Synopsis

Receptor for phage PIK specific for Pseudomonas aeruginosa strain PAO1 was studied. Phage PIK was strongly inactivated by lipopolysaccharide (LPS) in vitro, exhibiting a PhI50 of 4.8 micrograms/ml. Further it was noted that this inactivation by LPS was reduced to 50% by several mono- and disaccharides when tested in vitro. D-glucosamine, D-mannose and L-rhamnose were found to be most effective at the concentration of 0.045 M, 0.25 M and 0.35 M respectively. This suggests the possibility that phage PIK receptor in LPS contains D-mannose, L-rhamnose and D-glucosamine. Either one of the former two could be located at a terminal position alpha-linked to the adjacent residue or located internally in the polysaccharide chain linked through its C-4 position. A theoretical approach to the interpretation of phage cell interaction was also investigated.