Studies on the relationship of viral infections to leukemia in mice. III. Failure to demonstrate acceleration of leukemia in mature AKR mice by cell-free brain filtrates from leukemic animals of the same strain

✍ Scribed by Robert E. Carter; Mila I. Pierce

• Publisher: John Wiley and Sons
• Year: 1961
• Tongue: English
• Weight: 504 KB
• Volume: 14
• Category: Article
• ISSN: 0008-543X
• DOI: 10.1002/1097-0142(199005/06)14:3<496::aid-cncr2820140307>3.0.co;2-n

✦ Synopsis

demonstration of the induction T of rodent leukemia in mature mice by cell-free materials was reported by Schwartz and coworkers.11 I n the original experiment,ll a n acceleration of the development of leukemia in the high leukemic incidence strain of AKR mice was demonstrated by inoculation of mice at age 4 to 16 weeks with filtrates prepared from the brains of mice of the same strain that developed spontaneous leukemia. Subsequently, the induction of leukemia in mice of low leukemia incidence (Swiss and C3H strains) was demonstrated. Swiss mice were injected when more than 4 weeks of age with brain filtrates prepared from DBA mice carrying a cell-transmitted leukemia originating spontaneously in a Swiss mouse. T h e recipients developed leukemia within a few weeks after inoculation.8 Similarly, various C3H strains, injected with brain filtrates from C3H mice carrying a cell-transmitted leukemia originating spontaneously in a single C3H mouse, developed leukemia in a high percentage of cases.lO These experiments were singular in 2 respects, (1) the leukemogenic agent was transmitted by brain filtrates from the leukemic animals but not from cell-free filtrates of tumor cells or involved lymph nodes in certain of the experiments, and (2) the filtrates produced leukemia in animals inoculated when more than 4 weeks of age. Other investigators2, 4-6 have demonstrated leukemogenic or oncogenic effect with cell-free filtrates of leukemic mouse tissue only by inoculation of mice during the early newborn period. Friend3 reported an unusual type of transmis-