Studies on the possible chemical, immunochemical and morphological differences at the cell surfaces of immunosensitive and immunoresistant, moloney virus-induced, lymphoma cell-lines
✍ Scribed by Hasi R. Das; John F. Codington; Lorna A. Lampert; Mary D. Maxfield; Roger W. Jeanloz; Scott C. Miller; George Klein; Tina Dalianis
- Book ID
- 102991045
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 816 KB
- Volume
- 120
- Category
- Article
- ISSN
- 0008-6215
No coin nor oath required. For personal study only.
✦ Synopsis
Comparison was made of several cell-surface parameters in the immunosensitive, Moloney virus-induced, mouse lymphoma, YAC, and its immunoresistant variant, YACIR . The characteristics of the two cell lines appeared to be similar by most of the criteria employed . The poly(acrylamide)-gel electrophoresis (with sodium dodecyl sulfate) patterns, after staining with Coomassie Brilliant Blue, of detergent-solubilized materials, appeared to be identical . After elution from a gelfiltration column, no major differences were observed in the protein profiles of material cleaved from viable cells by proteolysis . Scanning and transmission electron microscopy revealed no major differences between the YAC and YACIR cells . The concentration of the lectins, Ricinus communis agglutinin, concanavalin A, wheat-germ agglutinin, and Solanum tuberosum (potato) agglutinin, required to agglutinate viable cells of the two lines were not significantly different . Neither cell was agglutinated by the lectins from Dolichos biflorus or Vicia graminea . Significant differences were, however, observed in the concentrations of lectin from *Dedicated to Professor Elvin A . Kabat .