Studies on the nature and localization of acid phosphatase in normal and lens-regenerating urodele eyes. II. Biochemical assay

✍ Scribed by Neil R. Miller; Nancy S. Rafferty

• Publisher: John Wiley and Sons
• Year: 1969
• Tongue: English
• Weight: 717 KB
• Volume: 129
• Category: Article
• ISSN: 0362-2525
• DOI: 10.1002/jmor.1051290307

✦ Synopsis

Biocheniical assay of acid phosphatase in normal and lens-regenerating eyes of the urodele Diemictylus virickscens, using p-nitrophenyl phosphate as substrate, demonstrates both soluble and lysosomal fractions of the enzyme. While the specific activity of the soluble fraction remains unchanged during lens regeneration, the lysosomal fraction shows four distinct rises i n specific activity during the thirty-day regeneration period studied. These peak activities on the second, eighth, fifteenth, and twenty-second days post-lentectomy apparently correspond to Iysnsomal activity in the processes 01 wound healing, iris depigmentation, and lens differen tiation which occur during urodele lens regeneration. On the basis of biochemical and histochemical studies as well as observations of morphological changes in the urodele eye as lens regeneration proceeds, it is postulated that there is a significant correlation between these morphological changes and the level and localizaiion of the lysosonial acid hydrolases in the tissues in which the changes occur.