Studies on the kinetics and mechanism of reduction of flavodoxin from Peptostreptococcus elsdenii by sodium dithionite
β Scribed by Stephen G. Mayhew; Vincent Massey
- Book ID
- 115733220
- Publisher
- Elsevier Science
- Year
- 1973
- Weight
- 548 KB
- Volume
- 315
- Category
- Article
- ISSN
- 0005-2744
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## Abstract The binding of various flavins by apoflavodoxins from __P. elsdenii__ and __A. vinelandii__ has been studied by the temperatureβjump technique using fluorescence detection. __P. elsdenii__ apoflavodoxin interacts only with flavins possessing 5 carbon atoms in the N(10) side chain and a
The reaction of 2,6-dichlorophenolindophenol (DCPI) and dithionites is studied kinetically by applying the stopped-flow technique. Reaction rate constants are given for the pH range 1.30-6.80. The reaction was found to follow first-order kinetics with respect to each of the reactants. For pH 3.97,5.