Studies on the genomic heterogeneity of Micrococcus luteus strains by macro-restriction analysis using pulsed-field gel electrophoresis
✍ Scribed by O. Murayama; M. Matsuda; J. E. Moore
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 54 KB
- Volume
- 43
- Category
- Article
- ISSN
- 0233-111X
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✦ Synopsis
Abstract
Macro‐restriction analysis by means of double digestion using __Dra__I and __Vsp__I demonstrated that they cleaved the genomic DNAs from Micrococcus luteus JCM1464^T^, JCM3347, and JCM3348 into four to five fragments in a distinguishable manner by pulsed‐field gel electrophoresis (PFGE). Separate digestion with __Dra__I and __Vsp__I cleaved the genomic DNA from M. luteus ATCC9341 into a relatively limited number of restriction fragments (six pieces). __Ssp__I and __Xba__I cleaved the genomic DNA from each of the four strains into restriction fragments in a distinctly different and distinguishable manner.
Thus, PFGE profiles after digestion with these four restriction enzymes that recognize six specific base sequences demonstrated the heterogeneity at the whole genomic DNA level among the four strains of M. luteus.
The size of the genomic DNA of M. luteus ATCC9341 was estimated to be approximately 2.3 Mb by the summing the lengths of the restriction fragments generated by each three restriction enzymes (__Dra__I, __Ssp__I, and __Vsp__I) and averaging the results.