Studies on the extraction and back-extraction of a recombinant cutinase in a reversed micellar extraction process

✍ Scribed by M. G. Carneiro-da-Cunha; J. M. S. Cabral; M. R. Aires-Barros

• Publisher: Springer
• Year: 1994
• Tongue: English
• Weight: 639 KB
• Volume: 11
• Category: Article
• ISSN: 1615-7605
• DOI: 10.1007/bf00369631

✦ Synopsis

This work deals with the extraction and backextraction of a recombinant cutinase using AOT reversed micelles in isooctane. The effect of pH, ionic strength, AOT concentration and temperature on the extraction and back-extraction of the cutinase was investigated. High extraction (97%) of the cutinase was achieved at pH 7.o with a 5o mM Tris-HC1 buffer solution containing loo mM KC1, but a low activity was detected in the reversed micellar phase. At pH 9.o, cutinase was extracted (75%) to the reversed micelles with higher activity. Cutinase was recovered (50%) from a reversed micellar phase (loo mM AOT/isooctane) into a 5o mM Tris-HC1 buffered solution at pH 9.o with loo mM KC1, and zo~ Protein and cutinase activity global yields of 38 and 45%, respectively, were obtained for the global process, extraction and backextraction steps, using low ionic strength, pH 9.o, loo mM AOT and zo~ 1