Studies on the energy metabolism of opossum (didelphis virginiana) erythrocytes—VI. De novo purine nucleotide biosynthesis is limited to the final steps of the pathway in vitro

Studies on the energy metabolism of opos

Studies on the energy metabolism of opossum (Didelphis virginiana) erythrocytes: V. Utilization of hypoxanthine for the synthesis of adenine and guanine nucleotides in vitro

✍ N. C. Bethlenfalvay; J. C. White; E. Chadwick; J. E. Lima 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 567 KB

High pressure liquid radiochromatography was used to test the ability of opossum erythrocytes to incorporate tracer amounts of [ G 3 H I hypoxanthine (Hy) into ['HI labelled triphosphates of adenine and guanine. In the presence of supraphysiologic (30 mM) phosphate which is optimal for PRPP synthesi