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Studies on the effect of NAD(H) on nitrogenase activity inRhodospirillum rubrum

✍ Scribed by Abdelhamid Soliman; Stefan Nordlund

Publisher
Springer
Year
1992
Tongue
English
Weight
548 KB
Volume
157
Category
Article
ISSN
0302-8933

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✦ Synopsis

The effect of NAD(P) and analogs of this nucleotide on nitrogenase activity in Rhodospirillum rubrum has been studied. Addition of NAD+ to nitrogen fixing Rsp. rubrum leads to inhibition of nitrogenase. NADP+ has the same effect but NADH or analogs modified in the nicotinamide portion do not cause inhibition. In contrast to ammonium ions, addition of NAD+ leads to inhibition of nitrogenase in cells that have been N-starved under argon. The inhibitory effect of NAD+ is more pronounced at lower light intensities. Addition of NAD+ also leads to inhibition of glutamine synthetase, a phenomenon also occurring when "switch-off" is produced by the addition of effectors such as ammonium ions or glutamine. It is also shown that NAD+ is taken up by Rsp. rubrum cells.

πŸ“œ SIMILAR VOLUMES

The role of glutamine synthetase in the
The role of glutamine synthetase in the regulation of nitrogenase activity (β€œswitch off” effect) inRhodospirillum rubrum
✍ Gunnar Falk; Bo C. Johansson; Stefan Nordlund πŸ“‚ Article πŸ“… 1982 πŸ› Springer 🌐 English βš– 298 KB

We have studied the changes in the activities of both nitrogenase ("switch off") and glutamine synthetase in Rhodospirillum rubrum upon addition of ammonium ions or glutamine to nitrogen fixing cultures. Both activities decrease drastically and return in a parallel manner when added ammonia is metab