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Studies on the development of mouse embryosin vitro. I. The effect of osmolarity and hydrogen ion concentration

✍ Scribed by Brinster, Ralph L.


Publisher
John Wiley and Sons
Year
1965
Tongue
English
Weight
696 KB
Volume
158
Category
Article
ISSN
0022-104X

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Studies on the development of mouse emby
✍ Brinster, Ralph L. πŸ“‚ Article πŸ“… 1965 πŸ› John Wiley and Sons 🌐 English βš– 659 KB

The effect of various possible energy sources on the development in vitro of two-cell mouse ova into blastocysts was examined. Energy for develop- ment of two-cell mouse ova could be supplied by lactate, pyruvate, oxaloacetate, or phosphoenolpyruvate. Compounds such as glucose, fructose, ribose, glu

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## Abstract The optimal oxygen tension for development of preimplantation mouse embryos to the blastocyst stage in vitro was found to be between 2.5% and 5%. One‐ and two‐cell embryos had a more sharply defined range of oxygen tension capable of supporting development than 8‐cell and morula stages.

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The present study was designed to examine the effects of changes in Ca2+ concentration in a culture medium on the development of 11day mouse embryos (plug day = day 0). The embryos were individually cultured for 24 hours by rotating bottles using a culture medium of 25% rat serum and 75% Waymouth's

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Prolongation of the life span has been demonstrated to result from low temperatures while high temperatures shorten it. This action of temperature has been demonstrated by several workers, among them Loeb and Northrup ( '17) in the case of Drosophila, and Schwerdtfeger ( '38) with Melolontha. Retard